How are polyclonal antibodies produced in a rabbit?
Of the three standard options for rabbit polyclonal antibody production, this protocol is optimized to maximize titer and volume by ensuring that sufficient time is provided for full maturation of the secondary immune response and serum antibody production in each rabbit.
What happens if you give a rabbit a pregnancy test?
The ‘rabbit test’ for pregnancy didn’t actually mean that if the rabbit died, you were pregnant. In 1927, medical researchers found that not only is hCG present in the urine of pregnant women, but that female rabbits injected with urine containing hCG would, within a few days, display distinct ovarian changes.
When do you give a peptide antigen to a rabbit?
Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before day 0. Injection amounts are given for a conjugated peptide antigen (e.g., KLH) or protein immunogen; MAP-peptide antigens are injected at 0.5mg throughout. Protocol days are approximate (± 2 days).
How long does it take to make a custom rabbit antibody?
Injections are subcutaneous (SQ) as emulsions in Complete Freund’s Adjuvant (CFA) or Incomplete Freund’s Adjuvant (IFA); alternative adjuvants can be used if requested. Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before Day 0. Protocol days are approximate (± 2 days).
The ‘rabbit test’ for pregnancy didn’t actually mean that if the rabbit died, you were pregnant. In 1927, medical researchers found that not only is hCG present in the urine of pregnant women, but that female rabbits injected with urine containing hCG would, within a few days, display distinct ovarian changes.
Can a male rabbit get pregnant after being spayed?
At this point you will need to make sure they are separated from any males until your rabbit has been spayed so you can prevent any unwanted litters. Rabbits can also become pregnant again directly after having a litter, and they can become pregnant at any time of year.
What should I do if my Bunny is pregnant?
That’s a lot of bunnies! That said, in the case that you are caring for a pregnant rabbit or new mother, here are a few basic tips: Separate the male rabbit from the female so they cannot mate.
How old do female rabbits have to be to get pregnant?
In fact, female rabbits can become pregnant after just 12 weeks. However, it is better to avoid this, as their bodies are not fully ready to bear the physical strain of pregnancy. At the other end of the spectrum, female rabbits over 4 years old should not be mated. They are too old for pregnancy.
