What advantage does the 10X objective have over the 100x?
Low Power Objective (10x) The total magnification of a low power objective lens combined with a 10x eyepiece lens is 100x magnification, giving you a closer view of the slide than a scanning objective lens without getting too close for general viewing purposes.
Which objective lens is most commonly used to observe bacteria?
Generally, objective lenses of 60X or greater might use oil — and they certainly will by the time you reach 100X. Because oculars are typically 10X, oil is necessary for viewing bacteria at a magnification of 1000X.
What is total magnification of 10X 45x and 100x condenser lens?
The objective and ocular lenses are responsible for magnifying the image of the specimen being viewed. So for 10X objective and 10X ocular, Total magnification = 10 X 10 = 100X (this means that the image being viewed will appear to be 100 times its actual size).
Which of the following objective lens should be used first in microscopy?
ALWAYS use both hands when picking the microscope up and moving it from one place to another. 3. When focusing on a slide, ALWAYS start with either the 4X or 10X objective. Once you have the object in focus, then switch to the next higher power objective.
Why is oil used with the 100x objective?
The 100x lens is immersed in a drop of oil placed on the slide in order to eliminate any air gaps and lossof light due to refraction (bending of the light) as the light passes from glass (slide) → air → glass (objective lens). Immersion oil has the same refractive index of glass.
What is the shortest objective called?
scanning-power objective lens
After the light has passed through the specimen, it enters the objective lens (often called “objective” for short). The shortest of the three objectives is the scanning-power objective lens (N), and has a power of 4X.
What happens if you try to magnify it using 40x or 100x?
What happens to your image if you try to magnify it using 40x or 100x? It could blow up your iage if you do not adjust the stage accordingly.
What is the working distance for a 10x objective lens?
20.3 mm
Table 2 – Long Working Distance Objectives
| Designation | Magnification | Working Distance |
|---|---|---|
| ELWD | 100x | 2.0 mm |
| SLWD | 10x | 20.3 mm |
| SLWD | 20x | 20.5 mm |
| SLWD | 50x | 13.8 mm |
What is the lens in the eyepiece called?
Negative eyepieces have two lenses: the upper lens, which is closest to the observer’s eye, is called the eye-lens and the lower lens (beneath the diaphragm) is often termed the field lens.
What is the first lens you look through on a microscope?
Eyepiece Lens
Eyepiece Lens: the lens at the top that you look through. They are usually 10X or 15X power. Base: The bottom of the microscope, used for support Illuminator: A steady light source (110 volts) used in place of a mirror.
