What is toluidine blue used for?
Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity. Use of toluidine blue in tissue sections is done with the aim to highlight components, such as mast cells granules, mucins, and cartilage.
What is Ortho toluidine used for?
ortho-Toluidine is used as an intermediate in the synthesis of the large-volume herbicides, metolachlor and acetochlor, in the manufacture of more than 90 dyes and pigments (e.g. acid-fast dyestuffs, azo pigment dyes, triarylmethane dyes, sulfur dyes, and indigo compounds), and as an intermediate for synthetic rubber …
What does toluidine blue stain in bone?
Objectives. Staining with toluidine blue is a well-established procedure for the histological assessment of cartilaginous- and chondrogenic-differentiated tissues. Being a cationic dye, toluidine blue staining visualizes proteoglycans in a tissue because of its high affinity for the sulfate groups in proteoglycans.
How do you use toluidine blue dye?
Break the T-Blue swab handle, instilling dye into the attached swab. 2. Apply toluidine blue to areas of the external genitalia where injury has been noted prior to insertion of speculum in the female patient.
Why are stains useful in microscopy?
The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.
Why do neuronal bodies stain blue?
Because ammonium sulfide is a reducing agent, the stain is initially decolorized and the cell bodies appear blue only during fixation and dehydration. This makes differentiation somewhat difficult to control, and after dehydration it may be necessary to return to fixative for further differentiation.
What is o-Toluidine method?
Abstract. o-Toluidine, 6% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3% (w/v) trichloracetic acid. A stable green color develops after heating at 100° for 10 min., and the absorbance is determined at 630 or 635 mµ.
Is o-Toluidine toxic?
o-Toluidine is primarily used in the manufacture of dyes. o-Toluidine is highly toxic to humans when absorbed through the skin, inhaled as vapor, or swallowed. Acute (short-term) exposure of humans to o- toluidine affects the blood (i.e., methemoglobinemia), with clinical signs of central nervous system depression.
How do you remove blue toluidine?
On skin and clothing: wash skin with strong soap solution immediately. Rinse thoroughly. Contaminated clothing should be removed, dried and washed with strong soap solution or destroyed. It may be necessary to destroy shoes by burning [NB added personally: see below ].
What is purpose of staining?
What are the types of stains?
Seven Types of Stain
- Oil Stain. Oil stains are the most widely available and the type of stain most people think of when they think of stain.
- Varnish Stain. Varnish stains resemble oil stains in every way but one.
- Gel Stain.
- Lacquer Stain.
- Water-Soluble Dye Stain.
- Metal-Complex (Metalized) Dye Stain.
What staining is best for cytoplasm?
Haematoxylin and eosin (H&E) staining Haematoxylin and eosin staining is frequently used in histology to examine thin tissue sections. Haematoxylin stains cell nuclei blue, while eosin stains cytoplasm, connective tissue and other extracellular substances pink or red.
What is the principle of O tolidine test?
Principle. The proteins are first precipitated by tricholoroacetic acid. The glucose present in a protein free filtrate react with O-toluidine (primary aromatic amine) in a hot acidic medium to form a stable green colored complex, namely N-glycosamine. The presence of thiourea stabilizes the o-toluidine reaction.
How do you make o-toluidine?
o-Toluidine is produced industrially by nitration of toluene to give mixture of nitrotoluenes, favoring the ortho isomer. This mixture is separated by distillation. 2-Nitrotoluene is hydrogenated to give o-toluidine.
What is O-toluidine method?
How do you make a green stain fast?
- Deparaffinize slides and rehydrate to deionized water. Stain with 0.04% Toluidine Blue Solution for 10 min.
- Rinse gently with 3 changes of deionized water (30 sec each) Counterstain with 0.02% Fast Green solution for 3 min.
- Rinse gently with 2 changes of deionized water (30 sec each)
What is purpose of staining bacteria?
Introduction: Gram staining is a method commonly used to determine the chemical make up of the cell wall of bacteria. The cell wall can stain either positive or negative, depending on its chemistry. Knowing the chemical make up makes it easier to manipulate the bacteria for various purposes.
What are the three basic stains?
Basic stains, such as methylene blue, Gram safranin, or Gram crystal violet are useful for staining most bacteria.
What are the three kinds of stains?
There are three broad categories of biological stains:
- General or Routine Stains: Used to differentiate between the nucleus and the cytoplasm.
- Special Stains: These are used to demonstrate specific elements within the tissue, such as connective tissue, muscle, carbohydrates, lipids, pigments, and nerve tissue.
What are the purposes of staining bacteria?
